Modulation of enzymatic activities of vitamin A-metabolizing enzymes by cellular retinol-binding proteins

2015 Fiscal Year Final Research Report

• Project/Area Number: 25504001
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrated Nutrition Science
• Research Institution: Jikei University School of Medicine (2015); Akita University (2013-2014)
• Principal Investigator: Mezaki Yoshihiro 東京慈恵会医科大学, 医学部, 講師 (40431621)
• Co-Investigator(Kenkyū-buntansha): SENOO Haruki 秋田大学, 大学院医学系研究科, 教授 (90171355) ; YAMAGUCHI Noriko 秋田大学, 大学院医学系研究科, 准教授 (90251553) ; YOSHIKAWA Kiwamu 秋田大学, 大学院医学系研究科, 助教 (90400481)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ビタミンA / 星細胞 / 肝臓 / 小腸 / 酵素 / タンパク質

Outline of Final Research Achievements

Retinyl esters (REs) were quantified in cells transfected with lecithin:retinol acyltransferase (LRAT). The amount of REs was up-regulated in cells co-transfected with cellular retinol-binding protein (CRBP) II. However, prolonged cultivation resulted in decrease in the amount of REs. Deposition of REs into the lipid droplets in hepatic stellate cells and discharge of REs from intestinal absorptive cells are considered to be important for proper enzymatic activity of LRAT.

Free Research Field

生化学