Development of a reliable peptidomics technique using a novel chemical derivatization approach

2015 Fiscal Year Final Research Report

• Project/Area Number: 25513001
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: オミクス計測科学
• Research Institution: Kyoto University
• Principal Investigator: Miyagawa Hisashi 京都大学, (連合)農学研究科(研究院), 教授 (10219735)
• Co-Investigator(Renkei-kenkyūsha): MIYASHITA Masahiro 京都大学, 大学院農学研究科, 助教 (80324664)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ペプチド / 質量分析 / アミノ酸配列決定 / 化学修飾 / 抗菌性

Outline of Final Research Achievements

Peptide sequencing by mass spectrometry is known to be facilitated by chemical modification of the peptide N-terminus. In this study, we fist optimized the method for N-terminal selective modification and then investigated the modification structures suitable for Leu/Ile discrimination under high-energy CID conditions. In addition, we successfully identified three novel antimicrobial peptides from the scorpion Isometrus maculatus venom using the method developed in this study.

Free Research Field

生物有機化学