2015 Fiscal Year Final Research Report
Analysis of epigenetic regulation by visualizing S-adenosylmethionine metabolism in cancer cells using imaging mass spectrometry.
| Project/Area Number |
25513008
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
オミクス計測科学
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| Research Institution | Keio University |
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Principal Investigator |
Kubo Akiko 慶應義塾大学, 医学部, 講師 (50455573)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAMOTO Takehiro 慶應義塾大学, 医学部, 講師 (50383774)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | メタボロミクス / 質量分析イメージング |
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| Outline of Final Research Achievements |
In this study, we focused on S-adenosylmethionine (SAM) metabolism. SAM is the only substrate in which is used in all the methylation reaction in vivo. In an experimental model of xenograft transplantation of human colon cancer HCT116 cells in superimmunodeficient NOG mice, snap-frozen liver tissues containing metastatic tumors were examined by quantitative-imaging mass spectrometry. As reported previously, short hairpin CD44 RNA interference (shCD44) in cancer cells caused significant regression of tumor growth in the host liver. Under these circumstances, the CD44 knockdown suppressed polyamines (polyamines are biosynthesized from SAM), GSH and energy charges.
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| Free Research Field |
メタボロミクス
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