2013 Fiscal Year Final Research Report
Identification of SNPs on mature micro RNA to analyze origin of miRNA
| Project/Area Number |
25670369
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Hiroshima University |
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Principal Investigator |
CHAYAMA Kazuaki 広島大学, 医歯薬保健学研究院, 教授 (00211376)
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| Project Period (FY) |
2013-04-01 – 2014-03-31
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| Keywords | 肝臓学 / SNP / digital PCR / microRNA / transplantation |
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| Research Abstract |
Recent research made it clear that there are certain amount of circulating miRNA in serum, which present in serum associated with proteins such as AGO1 and AGO2. Such AGO-protein bound miRNA is quite stable and detectable by polymerase chain reaction after extraction from the serum samples and reverse transcription. We attempted to analyze organs which secrete each miRNA by analyzing circulating miRNA that is present in serum samples. To perform this analysis we tried to identify SNPs that is present in miRNA. We searched for such SNP in entire human genome analyzing human genome database and identified several candidate SNPs. Although we attempted to detect such miRNA with digital PCR, detection of small amount of miRNA was quite difficult. Further study is needed to detect such small amount of miRNA in serum samples.
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[Journal Article] Daclatasvir plus asunaprevir for chronic HCV genotype 1b infection2014
Author(s)
Kumada H, Suzuki Y, Ikeda K, Toyota J, Karino Y, Chayama K, Kawakami Y, Ido A, Yamamoto K, Takaguchi K, Izumi N, Koike K, Takehara T, Kawada N, Sata M, Miyagoshi H, Eley T, McPhee F, Damokosh A, Ishikawa H, and Hughes E
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Journal Title
Hepatology
Volume: (in press)
DOI
Peer Reviewed
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[Journal Article] Dual oral therapy with daclatasvir and asunaprevir for patients with HCV genotype 1b infection and limited treatment options2013
Author(s)
Suzuki Y, Ikeda K, Suzuki F, Toyota J, Karino Y, Chayama K, Kawakami Y, Ishikawa H, Watanabe H, Hu W, Eley T, McPhee F, Hughes E, and Kumada H
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Journal Title
J Hepatol
Volume: 58
Pages: 62-655
DOI
Peer Reviewed
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[Journal Article] Combination therapies with NS5A, NS3 and NS5B inhibitors on different genotypes of hepatitis C virus in human hepatocyte chimeric mice2013
Author(s)
Shi N, Hiraga N, Imamura M, Hayes C N, Zhang Y, Kosaka K, Okazaki A, Murakami E, Tsuge M, Abe H, Aikata H, Takahashi S, Ochi H, Tateno-Mukaidani C, Yoshizato K, Matsui H, Kanai A, Inaba T, McPhee F, Gao M, and Chayama K
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Journal Title
Gut
Volume: 62
Pages: 61-1055
DOI
Peer Reviewed
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[Journal Article] A nonsynonymous polymorphism in semaphorin 3A as a risk factor for human unexplained cardiac arrest with documented ventricular fibrillation2013
Author(s)
Nakano Y, Chayama K, Ochi H, Toshishige M, Hayashida Y, Miki D, Hayes C N, Suzuki H, Tokuyama T, Oda N, Suenari K, Uchimura-Makita Y, Kajihara K, Sairaku A, Motoda C, Fujiwara M, Watanabe Y, Yoshida Y, Ohkubo K, Watanabe I, Nogami A, Hasegawa K, Watanabe H, Endo N, Aiba T, Shimizu W, Ohno S, Horie M, Arihiro K, Tashiro S, Makita N, and Kihara Y
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Journal Title
PLoS Genet
Volume: 9
Pages: e1003364
DOI
Peer Reviewed
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[Journal Article] HLA-DQB1*03 confers susceptibility to chronic hepatitis C in Japanese : a genome-wide association study2013
Author(s)
Miki D, Ochi H, Takahashi A, Hayes C N, Urabe Y, Abe H, Kawaoka T, Tsuge M, Hiraga N, Imamura M, Kawakami Y, Aikata H, Takahashi S, Akuta N, Suzuki F, Ikeda K, Kumada H, Karino Y, Toyota J, Tsunoda T, Kubo M, Kamatani N, Nakamura Y, and Chayama K
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Journal Title
PLoS One
Volume: 8
Pages: e84226
DOI
Peer Reviewed
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[Journal Article] Autologous bone-marrow mesenchymal stem cell implantation and endothelial function in a rabbit ischemic limb model2013
Author(s)
Mikami S, Nakashima A, Nakagawa K, Maruhashi T, Iwamoto Y, Kajikawa M, Matsumoto T, Kihara Y, Chayama K, Noma K, Ochi M, Nishimura M, Tsuji K, Kato Y, Goto C, and Higashi Y
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Journal Title
PLoS One
Volume: 8
Pages: e67739
DOI
Peer Reviewed
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[Journal Article] Identification of a functional variant in the MICA promoter which regulates MICA expression and increases HCV-related hepatocellular carcinoma risk2013
Author(s)
Lo P H, Urabe Y, Kumar V, Tanikawa C, Koike K, Kato N, Miki D, Chayama K, Kubo M, Nakamura Y, and Matsuda K
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Journal Title
PLoS One
Volume: 8
Pages: e61279
DOI
Peer Reviewed
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[Journal Article] Genetic analyses reveal a role for vitamin D insufficiency in HCV-associated hepatocellular carcinoma development2013
Author(s)
Lange C M, Miki D, Ochi H, Nischalke H D, Bojunga J, Bibert S, Morikawa K, Gouttenoire J, Cerny A, Dufour J F, Gorgievski-Hrisoho M, Heim M H, Malinverni R, Mullhaupt B, Negro F, Semela D, Kutalik Z, Muller T, Spengler U, Berg T, Chayama K, Moradpour D, Bochud P Y, Hiroshima Liver Study G, and Swiss Hepatitis C C S G
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Journal Title
PLoS One
Volume: 8
Pages: e64053
DOI
Peer Reviewed
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[Journal Article] Genome-wide scan revealed that polymorphisms in the PNPLA3, SAMM50, and PARVB genes are associated with development and progression of nonalcoholic fatty liver disease in Japan2013
Author(s)
Kitamoto T, Kitamoto A, Yoneda M, Hyogo H, Ochi H, Nakamura T, Teranishi H, Mizusawa S, Ueno T, Chayama K, Nakajima A, Nakao K, Sekine A, and Hotta K
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Journal Title
Hum Genet
Volume: 132
Pages: 92-783
DOI
Peer Reviewed
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[Journal Article] Characterization of virologic escape in hepatitis C virus genotype 1b patients treated with the direct-acting antivirals daclatasvir and asunaprevir2013
Author(s)
Karino Y, Toyota J, Ikeda K, Suzuki F, Chayama K, Kawakami Y, Ishikawa H, Watanabe H, Hernandez D, Yu F, McPhee F, and Kumada H
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Journal Title
J Hepatol
Volume: 58
Pages: 54-646
DOI
Peer Reviewed
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[Journal Article] Glycemic variability is an independent predictive factor for development of hepatic fibrosis in nonalcoholic fatty liver disease2013
Author(s)
Hashiba M, Ono M, Hyogo H, Ikeda Y, Masuda K, Yoshioka R, Ishikawa Y, Nagata Y, Munekage K, Ochi T, Hirose A, Nozaki-Fujimura Y, Noguchi S, Okamoto N, Chayama K, Suganuma N, and Saibara T
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Journal Title
PLoS One
Volume: 8
Pages: e76161
DOI
Peer Reviewed
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[Book] 肝臓、ゲノタイプ3a型のC型急性肝炎の1例―IL28B遺伝子からの考察―2013
Author(s)
小林知樹,川上由育,平松憲,瀧川英彦,野中裕広,田中未央,苗代典昭,中原隆志,本田洋士,宮木大輔,長沖祐子,河岡友和,高木慎太郎,柘植雅貴,今村道雄,兵庫秀幸,相方浩,高橋祥一,茶山一彰
Total Pages
120-127
Publisher
一般社団法人日本肝臓学会
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