Molecular basis regulating differentiation of mesenchymal stem cells in mandibular condylar cartilage by microRNAs

2015 Fiscal Year Final Research Report

• Project/Area Number: 25670876
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Kyushu University
• Principal Investigator: TAKAHAHI Ichiro 九州大学, 歯学研究科(研究院), 教授 (70241643)
• Co-Investigator(Kenkyū-buntansha): TERAO FUMIE 九州大学, 大学院歯学研究院, 助教 (10510018)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 発生・分化 / microRNA / 下顎頭軟骨

Outline of Final Research Achievements

Microarray analysis using osteo-chondroprogenitor cells, putative mesenchymal stem cells in mandibular condylar cartilage and those in the primary cartilages in shoulder joint during embryonic stages has revealed that microRNA-200a (miR-200a) is one of the candidate microRNA which regulates the differentiation of secondary cartilage. We found that Dlx5 is a candidate target gene for miR-200a by microarray analysis. Reporter assay revealed that miR-200a inhibited the promoter activities of Dlx-5. Further, miR-200a reduced the production of Dlx5 protein in ATDC5 cells. Thus, it has been suggested that miR-200a regulates the differentiation of mandibular condylar cartilage cells through inhibition of Dlx5 production.

Free Research Field

医歯薬学