In vitro induction of totipotency from somatic cells in mice

2016 Fiscal Year Final Research Report

• Project/Area Number: 25712030
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Animal production science
• Research Institution: Institute of Physical and Chemical Research (2013, 2016); University of Yamanashi (2014-2015)
• Principal Investigator: Ohinata Yasuhide 国立研究開発法人理化学研究所, 統合生命医科学研究センター, 研究員 (70415107)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 胚性幹細胞 / 栄養膜幹細胞 / 胚盤胞

Outline of Final Research Achievements

High quality mouse naive ES/iPS cells can readily form tetraploid chimeras, which almost consist of donor pluripotent stem cells; however, these stem cells are not capable of developing embryos without using early embryos as host. To date, reconstruction of artificial embryos by stem cells in vitro has not been achieved. Progress has largely been impeded by our present lack of understanding and techniques. Within the research period, we reported novel pluripotent stem cells (intermediate pluripotent stem cells, INTPSCs) that improve the properties of stem cells, are maintained FGF dependently and have chimeric contribution ability and germline transmission ability. We also reported that establishment of trophoblast stem cells under chemically defined culture conditions.

Free Research Field

初期発生