Strategic isolation culture of ectosymbiotic methanotroph by engineering innovation

2014 Fiscal Year Final Research Report

• Project/Area Number: 25840166
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ecology/Environment
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: WATSUJI Tomo-o 独立行政法人海洋研究開発機構, 深海・地殻内生物圏研究分野, 研究員 (50409091)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 深海外部共生 / メタン酸化細菌 / メタン添加飼育

Outline of Final Research Achievements

Shinkaia crosnieri in deep-sea hydrothermal vents in the Okinawa Trough has ingested epibiotic methanotrophs and thioautotrophs associated with setae in the ventral aspect. Therefore, we tried to rear S. crosnieri in an aquarium with added methane and succeeded in the rearing of S. crosnieri, which kept the association with epibiotic methanotrophs, during more than one year. We tried to isolate the epibiotic methanotrophs associated with S. crosnieri in the methane-added aquarium. But the epibiotic methanotrophs were not isolated in pure culture.

Free Research Field

極限環境共生学