Does electrical stimulation enhance the migration of transplanted cells in stroke model of rats? : Close up the mechanisms of electrical stimulation and search for the road to clinical application.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25861277
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurosurgery
• Research Institution: Okayama University
• Principal Investigator: Yasuhara Takao 岡山大学, 医歯(薬)学総合研究科, 講師 (50457214)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 細胞移植 / 電気刺激 / 細胞遊走能 / 細胞分化 / 脳梗塞 / パーキンソン病 / 幹細胞 / 再生医療

Outline of Final Research Achievements

Electrical stimulation was performed through epidural electrode on infarcted cerebral cortex in a stroke model of rats using middle cerebral artery occlusion. Mesenchymal stem cells were transplanted into contralateral cerebral cortex after fluorescent labeling. After electrical stimulation for 2 weeks, cell proliferation, differentiation and migration were explored. The cell migration to the cerebral infarct was significantly enhanced by electrical stimulation, although there was no significant difference in cell proliferation and differentiation of transplanted mesenchymal stem cells. Bromodeoxyuridine labeling revealed that endogenous neural stem cells were more proliferated with more neural differentiations.

Free Research Field

医歯薬学、外科系臨床医学、脳神経外科学