2015 Fiscal Year Final Research Report
Analyses of glucagon related peptides in amacrine cells of eyes induced form deprivation myopia
Project/Area Number |
25861622
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Ophthalmology
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Research Institution | Tokyo Medical and Dental University |
Principal Investigator |
Nagaoka Natsuko 東京医科歯科大学, 医学部附属病院, 医員 (30626271)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
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Keywords | 実験近視 / アマクリン細胞 / グルカゴン / ラット / GIP / VIP / 視覚刺激遮断 |
Outline of Final Research Achievements |
We analyzed the change of the expression of glucagon family in Amacrine cells of the retina in the progression of myopia using rat experimental myopia model. Immunohistochemical examinations showed GIP and VIP which were members of glucagon family in Amacrine cells in the retina both in the normal control and the myopia induced eyes. We analyzed the expression level of the mRNA of GIP and VIP by RT-PCR, and GIP and VIP protein expression amount by Western blotting, and they showed decreased expression of mRNA of GIP and VIP, GIP and VIP protein in myopia induced eyes compared with the normal control eyes. These substances were suggested to be associated with myopia progression. These results are expected to be useful for developing a therapy to inhibit the progression of myopia.
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Free Research Field |
強度近視
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