Immuno-genetic approaches for investigation of pathogenesis of new disease entity "IgG4-related disease"

2014 Fiscal Year Final Research Report

• Project/Area Number: 25870087
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Collagenous pathology/Allergology; Pathobiological dentistry/Dental radiology
• Research Institution: University of Tsukuba
• Principal Investigator: TSUBOI Hiroto 筑波大学, 医学医療系, 講師 (80580505)
• Co-Investigator(Renkei-kenkyūsha): NAKAI Yuji 弘前大学, 食料科学研究所, 教授 (10321788)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: IgG4関連疾患 / シェーグレン症候群 / DNAマイクロアレイ / 口唇唾液腺

Outline of Final Research Achievements

The gene expressions in LSGs were compared between IgG4-RD (N=5), SS (N=5), and HC (N=3) by DNA microarray. Clustering by principal component analysis (PCA), and pairwise comparison between IgG4-RD and SS were performed. We conducted validation by qPCR for identified candidate genes among up-regulated DEGs in IgG4-RD using RNA isolated from LSGs of IgG4-RD (N=9), SS (N=10), and HC (N=4) other than patients examined by DNA microarray. The expression of CCL18 and expressing cells in LSGs were compared between IgG4-RD, SS, and HC by immunofluorescence (IF) staining. Gene expression patterns in IgG4-RD, SS, and HC were quite different in PCA. 1321 up-regulated DEGs and　1320 down-regulated DEGs in IgG4-RD compared with SS were identified in pairwise comparison. We validated significantly higher expression of CCL18 in LSGs of IgG4-RD than in SS and HC (P<0.05) by qPCR. IF staining clarified that many CD68 positive macrophages expressed CCL18 in LSGs of IgG4-RD whereas not in SS and HC.

Free Research Field

臨床免疫学