2016 Fiscal Year Final Research Report
Lipid analysis of microenvironment in hepatocellular carcinoma using imaging mass spectrometry
| Project/Area Number |
25870302
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
Tumor biology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | 大腸癌 / 質量顕微鏡 / CTOS / 癌微小環境 / ホスファチジルイノシトール |
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| Outline of Final Research Achievements |
In this study,we aimed to seek out the cancer cell-autonomous acquisition of cancer outer edge-characterizing lipids in colorectal cancer by analysing phospholipids in CTOSs derived from colorectal cancer patients with matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS). A signal at m/z 885.5 in negative ion mode was detected specifically at the surface regions. The signal was identified as an arachidonic acid (AA)-containing phosphatidylinositol (PI), PI(18:0/20:4), by tandem mass spectrometry analysis. Quantitative analysis revealed that the amount of PI(18:0/20:4) in the surface region of CTOSs was two-fold higher than that in the medial region. Finally, PI(18:0/20:4) was enriched at the cancer cells/stromal interface in colorectal cancer patients. These data imply a possible
importance of AA-containing PI for colorectal cancer progression, and suggest cells expressing AAcontaining PI as potential targets for anti-cancer therapy.
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| Free Research Field |
肝胆膵悪性腫瘍
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