Analysis of sphingolipid changes in the aging of bone marrow stromal cells in a mouse hematopoietic system

2014 Fiscal Year Final Research Report

• Project/Area Number: 25893269
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Kanazawa Medical University
• Principal Investigator: OGISO Hideo 金沢医科大学, 医学部, 特定講師 (30466734)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Keywords: 細胞膜 / 脂質ラフト / リピドミクス

Outline of Final Research Achievements

We developed an approach for the comprehensive analysis of sphingolipids, glycerophospholipids and diacylglycerol molecular species in a biological sample, using LC-MS. In addition, we applied a cationic colloidal silica-bead method to analyze plasma membrane lipids from monolayer cells cultured in a 10-cm dish. The detergent-resistant fraction from the silica-bead coated membrane was analyzed to evaluate the microdomain lipids. Stromal cells from mouse bone marrow have an ability to maintain hematopoietic stem cells, but this ability decreases by aging in a process differentiating to fat cells. In this study, we analyzed some changes in lipids of membrane microdomains caused during differentiation of PA6 cells, one of the stromal cell lines, to fat cells. Result showed that ceramide level in the microdomain was elevated during the differentiation. This approach will facilitate future comprehensive analyses of membrane microdomain-associated responses in a hematopoietic system.

Free Research Field

生体成分の分析化学