Development of the methods that inhibits staphylococcal biofilm formation.

2017 Fiscal Year Final Research Report

• Project/Area Number: 26293100
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: Jikei University School of Medicine
• Principal Investigator: Mizunoe Yoshimitsu 東京慈恵会医科大学, 医学部, 教授 (20157514)
• Co-Investigator(Kenkyū-buntansha): 杉本 真也 東京慈恵会医科大学, 医学部, 講師 (60464393) ; 田嶌 亜紀子 東京慈恵会医科大学, 医学部, 講師 (70317973) ; 奥田 賢一 東京慈恵会医科大学, 医学部, 助教 (70624245) ; 岩瀬 忠行 東京慈恵会医科大学, 医学部, 助手 (80385294)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: バイオフィルム / 黄色ブドウ球菌 / バイオフィルム阻害剤 / Eap / SasG / 細胞壁タンパク質

Outline of Final Research Achievements

The combined deletion of Eap and SasG reduced biofilm biomass of Staphylococcus aureus, whereas single deletion did not. Combined deletion of Eap and SasG decreased both roughness and thickness. The pathogenicity of ΔEap ΔSasG was significantly decreased. Our findings highlight the relationship between Eap and SasG in S. aureus biofilm formation and pathogenesis. High-throughput screening identified norgestimate (NGM) as an inhibitor of biofilm formation by staphylococcal strains, including MRSA. NGM inhibited the production of extracellular matrix components that are important for biofilm formation, thereby inhibiting biofilm formation by clinical isolates with diverse matrix components. S. aureus caused biofilm dispersal by nuclease production and this depended on environmental pH. Dispersed bacteria showed highly virulence than planktonic bacteria in vitro and in vivo. Dispersed bacteria decreased phagocytosis by PMN and caused a lethal infection in mouse within 24 h.

Free Research Field

細菌学, 分子生物学