Regulation and analysis of tooth and salivary gland morphogenesis

2016 Fiscal Year Final Research Report

• Project/Area Number: 26293433
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Tohoku University
• Principal Investigator: FUKUMOTO EMIKO 東北大学, 歯学研究科(研究院), 大学院非常勤講師 (10264251)
• Co-Investigator(Kenkyū-buntansha): 福本 敏 東北大学, 歯学研究科(研究院), 教授 (30264253) ; 齋藤 正寛 東北大学, 歯学研究科(研究院), 教授 (40215562) ; 阪井 丘芳 大阪大学, 歯学研究科(研究院), 教授 (90379082) ; 保住 建太郎 東京薬科大学, 薬学部, 講師 (10453804)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 歯の発生 / 形態形成 / 唾液腺 / 再生

Outline of Final Research Achievements

To regenerate of tooth and salivary gland, it is important to identify the gene associated with the regulation of morphogenesis. However, it has never clearly understood the morphogenesis of tooth and salivary gland. Here, we identified the genes specifically expressed in tooth germ using microarray gene screening.
Mst1 is one of the Hippo pathway molecule expressed in embryonic tooth germ. Over expression of Mst1 in dental epithelial cells showed inhibition of proliferation. On the other hand, suppression of Mst1 expression using siRNA showed enhanced proliferation. Further, knockdown of Mob1 in mice, which is down stream molecule of Mst1, showed enhanced tooth size. Moreover, we identified the regulation of tooth width by NF-kB singaling molecule, NIK and p50. Further, GDF5 down-regulated enamel formation. These information is useful for establish the regeneration of tooth and salivary gland.

Free Research Field

小児歯科