2016 Fiscal Year Final Research Report
The elucidation of mechanisms of the cell death in SSCs induced by radiation
| Project/Area Number |
26340021
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 精子幹細胞 / 活性酸素 |
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| Outline of Final Research Achievements |
Exposure to ionizing radiation is known to have a strong influence H2O in vivo and induces reactive oxygen species (ROS). ROS makes severe DNA damages. In our study, we identified two molecules involved in the resistance to radiation in spermatogonial stem cells(SSCs). In consequence, we found that Nox3 and Bcl6b are novel molecules involved in the self renewal of SSCs. Activation of Mapk14 or Mapk7 increased the expression of Nox1 and ROS producing. Transplantation of Mapk14 or Mapk7 knocking out SSCs to donor testis resulted in a decrease in the number of colonies of SSCs. These results reveal that MAPK14-MAPK7-BCL6B pathway plays a crucial role in the self renewal of SSCs associated with ROS producing.
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| Free Research Field |
細胞生物学
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