The search of food component which shows the improvement of insulin resistance

2016 Fiscal Year Final Research Report

• Project/Area Number: 26350131
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Eating habits
• Research Institution: Kanagawa Institute of Technology
• Principal Investigator: Kiyose Chikako 神奈川工科大学, 応用バイオ科学部, 教授 (50272745)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 食品成分 / ビタミンＥ / インスリン抵抗性改善 / C2C12細胞 / KKAyマウス

Outline of Final Research Achievements

The aim of this study is the search of food component which improve insulin resistance. First, I tried a construction of insulin resistance cell using C2C12 cells, skeletal muscle cell of mouse. Consequently, I succeeded in the construction of the insulin resistance cell using palmitate. Next, I investigated the effects of vitamin E analogs on the improvement of insulin resistance in these insulin resistance cells. As the results of experiment, I found that delta-tocopherol can improve the phosphorylation of Akt in insulin resistance cells. Secondly, I investigated the effects of delta-tocopherol on the insulin resistance in KKAy mice, a diabetes model mice. The expressions of IRS-1 and IRS-2 mRNA in liver of H group were significantly lower than those of C group. But, there were no significant differences in the expressions of IRS-1 and IRS-2 mRNA between H group and delta-Toc group. Therefore, delta-tocopherol could not show an improvement effect of insulin resistance in KKAy mice.

Free Research Field

食品機能学