2016 Fiscal Year Final Research Report
Elucidation of dedifferentiation mechanism by transcriptome analysis
| Project/Area Number |
26430185
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
Machida Masayuki 独立行政法人産業技術総合研究所, 生物プロセス研究部門, 総括研究主幹 (30358006)
Kokubo Hiroki 広島大学, 大学院医歯薬保健学研究院, 講師 (10270480)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ゼブラフィッシュ / 再生 / トランスクリプトーム解析 |
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| Outline of Final Research Achievements |
Some vertebrates such as teleosts and amphibians can regenerate their lost appendages, whereas mammalians have limited regeneration ability. In animals that have regenerative ability, the restart of cell proliferation occurs during regeneration. Although this phenomenon is well known as “dedifferentiation”, the mechanism of this dedifferentiation remains elusive. To elucidate this dedifferentiation mechanism, I tried to screen genes that are expressed in the dedifferentiated proliferative cells during zebrafish fin regeneration by using next-generation sequencing. I identified the 132 genes that are up-regulated their expression more than twice after fin amputation compared with those before fin amputation. I am planning to analyze the function of these genes in dedifferentiation processes.
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| Free Research Field |
分子生物学
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