2016 Fiscal Year Final Research Report
A novel effect of HGF: mechanisms of irreversible arrest of cancer cell proliferation
| Project/Area Number |
26440048
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 肝細胞増殖因子 / 細胞増殖抑制 / HepG2細胞 / 癌 / エピゲノム / 遺伝子発現 |
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| Outline of Final Research Achievements |
Hepatocyte growth factor HGF has an inhibitory effect on the proliferation of human HepG2 hepatoma cells. With our study on HGF, we found its novel effect, irreversible arrest of cancer cell proliferation. In this study, we addressed mechanisms of the novel effect, and revealed following findings. (1) HepG2 cells, in which irreversible arrest of cell-proliferation can be induced with HGF, express Oct3/4, Sox2 and Klf4 genes, but none of them are concerned with the novel effect of HGF. (2) p57KIP2 is upregulated after 24 hours of HGF treatment, suggesting its involvement in the effect. (3) The novel effect of HGF requires downregulation of H3K9me3 and upregulation of histone methylation enzyme, G9a. (4) In accordance with the irreversible arrest of cell proliferation with HGF, the situation of cell differentiation can be altered. (5) Comprehensive analysis showed some genes, which expression is different between reversible arrest and irreversible arrest of HepG2 cells induced by HGF.
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| Free Research Field |
細胞周期制御、シグナル伝達制御、細胞増殖制御、癌
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