Development of a superior assay for activated platelet-derived microparticles by ELISA and elucidation of a mechanism of hypercoagulability.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460679
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Yamaguchi University
• Principal Investigator: OKANO Kozue 山口大学, 医学(系)研究科(研究院), 教授 (50160693)
• Research Collaborator: SHITAMOTO Kazuki
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 活性化血小板 / マイクロパーティクル / ELISA法 / 血小板関連抗体 / 抗AnnexinV抗体 / 採血条件 / 不整脈 / 抗凝固薬

Outline of Final Research Achievements

Activated-Platelet derived microparticle (aPLT-MP) has a strong influence on the blood clot formation and inflammatory, however, measuring method and mechanism of clot formation have not been clarified. We conducted a fundamental examination of an assay by ELISA using various platelet-related antibodies to determine aPLT-MP. Citrated plasma samples were obtained from 13 healthy volunteers, 72 patients with atrial fibrillation, 60 with hypertension, and 41 with diabetes mellitus.
The sensitivity and specificity of aPLT-MP varied according to the combination of platelet-related antibodies, and anti-AnnexinV polyclonal antibody for solid-phase and anti-GPIb monoclonal antibody for detector was the most suitable combination for aPLT-MP. There was no significant difference in PLT-MP values of volunteers between healthy and patients. Influencing factors to aPLT-MP values were arrhythmia and anticoagulant drugs.

Free Research Field

医歯薬学