Development of the methods for detection of diatoms utilizing silica binding abilities of DNA and proteins

2017 Fiscal Year Final Research Report

• Project/Area Number: 26460882
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Oita University
• Principal Investigator: Seo Yasuhisa 大分大学, 医学部, 助教 (80187830)
• Co-Investigator(Kenkyū-buntansha): 岸田 哲子 大分大学, 医学部, 教授 (50136793) ; 内田 智久 大分大学, 医学部, 助教 (70381035)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 溺死 / プランクトン / 珪藻 / 酵素染色 / ストレプトアビジン

Outline of Final Research Achievements

It was confirmed that some kinds of peroxidase-labeled streptavidin and IgG has abilities of non-specific adsorption to glassy matter. The methods of staining for diatom frustules were established. Then this staining method was applied to diatom tests that have been actually drowned victims. As a result, detection of the diatom frustules was easier, and the time to diagnose drowning was greatly shortened.
In addition, we could submit a paper on novel species of diatom discovered from sample water obtained from a drowning site during this research. Subsequent research confirmed that this diatom was also found in a completely different water system in Oita prefecture, and expansion of the habitat was found. Currently, we are conducting comparative study on the genetic diversity of diatoms discovered from each water system.

Free Research Field

医歯薬学