An analysis of the mechanisms through which bacteria-derived bioactive molecules protect the intestine in enteritis models and primary cultured cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 26460956
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Asahikawa Medical College
• Principal Investigator: Fujiya Mikihiro 旭川医科大学, 医学部, 准教授 (80322915)
• Co-Investigator(Kenkyū-buntansha): 上野 伸展 旭川医科大学, 医学部, 特任講師 (30436000) ; 稲場 勇平 旭川医科大学, 大学病院, その他 (30447099) ; 盛一 健太郎 旭川医科大学, 医学部, 講師 (70455715)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 腸内細菌 / プロバイオティクス / 炎症性腸疾患 / 消化器癌

Outline of Final Research Achievements

We previously proposed that probiotic-derived CSF and polyphosphate have protective effects in the intestine. This study investigated the functional mechanisms of these molecules. CSF was transported by OCTN2 and decreased the expression of inflammation-related cytokines, including IL-6, 7 and CXCL-1. Polyphosphate formed a complex with integrin b1 and caveolin-1 which was taken by endocytosis. It then activated p38 MAPK and augmented the intestinal barrier function, leading to the improvement of intestinal injury in a mouse model of enteritis. These results provide the detailed mechanisms of how probiotic-derived molecules protect the intestine and may contribute to the development of new drugs after the completion of preclinical and clinical studies.

Free Research Field

消化器内科