Efficacy of high density hydrogen water for the treatment of chemotherapy induced skin disease

2017 Fiscal Year Final Research Report

• Project/Area Number: 26461956
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Taguchi Tetsuya 京都府立医科大学, 医学(系)研究科(研究院), 教授 (80243260)
• Co-Investigator(Kenkyū-buntansha): 阪口 晃一 京都府立医科大学, 医学(系)研究科(研究院), 講師 (00405284)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 癌 / 化学療法 / 分子標的薬 / 抗癌剤 / 皮膚障害 / ヒト角化皮膚正常細胞株 / アポトーシス / ROS

Outline of Final Research Achievements

First of all, inhibitory effect of proliferation by afatinib, lapatinib and 5-FU to Ha-Cat (human keratinocyte) cell line was confirmed by WST-8 assay. And according to the FACS analysis, this proliferation suppression is likely to due to apoptosis instead of the cell cycle arrest. In addition, caspase independent mechanism was revealed by zVAD added pan-caspase inhibitory assay. Finally, according to the pan-Ros indicator and pan-ROS scavenger assay, this cell death was thought to be due to ROS. But we could not identify the specific type of ROS using specific ROS indicator assay.

Free Research Field

癌化学療法と副作用対策