The functional analysis of spindle body using the human ovum of each generation to be removed during surgery and nuclear transplantation.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462473
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Iwate Medical University (2016); Akita University (2014-2015)
• Principal Investigator: KUMAGAI JIN 岩手医科大学, 医学部, 教授 (60333936)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 卵子 / 蛍光免疫染色 / 卵子の輸送 / 電界撹拌

Outline of Final Research Achievements

Using non-contact alternating-current electric-field mixing of microdroplets, we developed the economical fluorescence immunostaining of the human ovum with time efficiency. We could stain the mouse ovum by this method using the anti-tubulin antibody which was the first antibody by 16,000 times dilution that 80 times as large as recommended dilution and could shorten discrimination time until five minutes. It is expected the saving precious antibodies and shortening the staining time in a future study.
On the other hand, concerning the human ovum transportation, in vitro maturation rate of human ovum significantly decreased in the immature ovum transportation case of 250km between Akita and Sendai, but improved it by stabilizing a vapor phase using a cell porter in a transportation container. This result is expected to bring the application to the ovum freeze of women with malignant tumor.

Free Research Field

生殖内分泌学