Development of a selective detection method of infectious viruses using pre-incubation with bacteria

2015 Fiscal Year Final Research Report

• Project/Area Number: 26550081
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Environmental risk control and evaluation
• Research Institution: The University of Tokyo
• Principal Investigator: KATAYAMA Hiroyuki 東京大学, 工学(系)研究科(研究院), 准教授 (00302779)
• Co-Investigator(Renkei-kenkyūsha): Kurisu Futoshi 東京大学, 大学院工学系研究科, 准教授 (30312979)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: ウイルス / PCR / 不活化

Outline of Final Research Achievements

PCR based detection cannot distinguish between infectious and non-infectious viruses. This research aimed to develop such a method to selectively remain infectious virion prior to PCR based detection. Various bacterial strains were applied to decompose naked viral RNA. However, no bacteria achieved a good performance to degrade RNA in a short time, determined by RT-PCR. Then,instead of bacteria, platinum reagents were tested for the same purpose. As a result, cis-DDP, one of platinum reagents, worked to remove RNA but remain intact virus RNA.

Free Research Field

環境工学