2017 Fiscal Year Final Research Report
Sex determining mechanism in streptophytes approached by the sex determining gene of Closterium peracerosum-strigosum-littorale complex
| Project/Area Number |
26650147
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biodiversity/Systematics
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| Research Institution | Japan Women's University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
坂山 英俊 神戸大学, 理学研究科, 准教授 (60391108)
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| Co-Investigator(Renkei-kenkyūsha) |
NISHIYAMA Tomoaki 金沢大学, 学際科学実験センター, 助教 (50390688)
TSUCHIKANE Yuki 日本女子大学, 理学部, 助教 (20434152)
ABE Jun 日本女子大学, 理学部, 学術研究員 (10424764)
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| Research Collaborator |
KAWAI Junko 日本女子大学, 理学部, 学術研究員 (30727673)
KOMIYA Ayumi 日本女子大学, 理学研究科, 大学院生
FUJIWARA Anri 日本女子大学, 理学研究科, 大学院生
KANDA Naho 日本女子大学, 理学研究科, 大学院生
TSUYUKI Natsumi 日本女子大学, 理学研究科, 大学院生
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | ミカヅキモ / 性決定 / ゲノム解読 / シャジクモ |
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| Outline of Final Research Achievements |
The CpMinus1 gene is a mt- genome-specific gene from the Closterium peracerosum-strigosum-littorale complex (C. psl. complex).
We developed the CpMinus1-expressing mt+ transformants. They showed a weak sexual reaction without mating partners, like as the case of homothallic strains, but tended to form zygotes with mt+ strain but not with mt- strain. The gene expression profile was drastically changed to that of wild mt- strains. We also obtained CpMinus1-knockout mutants from mt- background. The gene expression profile was quite similar to that of wild mt+ cells. We concluded that the CpMinus1 gene was a master gene for the sex determination in C. psl. complex. On the other hand, we could not find the CpMinus2 gene, which would be tightly linked to the CpMinus1 in the genome.
Through the Iso-seq data of sexual dimorphic Chara australis, two gene showing weak similarity to the CpMinus1 was found. One of them expressed only in the male strain, although the expression level was very low.
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| Free Research Field |
藻類生殖生理学
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