Identification of the target molecule of Ganoderma triterpenoid

2015 Fiscal Year Final Research Report

• Project/Area Number: 26660147
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Wood science
• Research Institution: Kyushu University
• Principal Investigator: shimizu kuniyoshi 九州大学, (連合)農学研究科(研究院), 准教授 (20346836)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 霊芝 / Ganoderma / トリテルペノイド / 標的タンパク質 / チューブリン / ケミカルバイオロジー / ganoderic acid DM / マンネンタケ

Outline of Final Research Achievements

The fungus Ganoderma lingzhi (Reishi, Mannentake, or Lingzhi) has been used for centuries in East Asia to treat various human diseases. Up to now, over 120 kinds of triterpenoids have been isolated from the genus Ganoderma. We have investigated anti-androgen effect and the inhibitory effects of osteoclastic differentiation of triterpenoids isolated from this mushroom in vitro. Ganoderic acid DM showed anti-androgenic activity which showed potent 5α-reductase inhibiting effect. Also, ganoderic acid DM inhibited the osteoclastic differentiation, which responding to receptor activator of NF-κB ligand (RANKL) and a tumor necrosis factor α (TNF-α). These results led us to investigate the target molecule of ganoderic acid DM by using affinity bead method. In this study, beta-tublin is identified as one of the target molecule of ganoderic acid DM in LNCaP cells. This result should be very helpful in elucidating the unsolved function of Ganoderma mushroom.

Free Research Field

森林圏生理活性科学、林産学、木材化学