Development of peptide inhibitors targeting ADAM10 to eradicate brain tumor-initiating cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670650
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurosurgery
• Research Institution: Aichi Cancer Center Research Institute
• Principal Investigator: IZAWA Ichiro 愛知県がんセンター(研究所), 腫瘍医化学部, 室長 (20311441)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: ADAM10 / テトラスパニン

Outline of Final Research Achievements

In this study, to utilize for the therapeutic methods of malignant gliomas, we tried to develop peptide inhibitors targeting ADAM10, a membrane-bound protease. We found that ADAM10 binds Tspan14, a member of TspanC8 subgroup of tetraspanin membrane proteins, via three domains, leading to the bending of the extracellular portions of ADAM10. We observed that the recombinant GST fusion proteins of these three domains of ADAM10 which were exogenously added to the culture media might be able to inhibit the activity of ADAM10 in the U251MG cell culture.

Free Research Field

分子医学