Development of intra-operaive intestinal ganglion cell detection system for Hirschsprung's disease and allied disorder using probe type confocal laser endomicroscopy

2016 Fiscal Year Final Research Report

• Project/Area Number: 26670765
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pediatric surgery
• Research Institution: Kagoshima University (2015-2016); Kyushu University (2014)
• Principal Investigator: Ieiri Satoshi 鹿児島大学, 医歯学域医学系, 教授 (00363359)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 機能性消化管疾患 / ヒルシュスプルング病 / 共焦点型内視鏡 / 神経節細胞 / 筋間神経叢 / ヒルシュスプルング病類縁疾患

Outline of Final Research Achievements

In preceding studies, we identified that some fluorescent agents cloud　visualization of the myenteric plexus (MP) with confocal laser endomicroscopy (CLE). In this study, we evaluated the technical feasibility of MP visualization using probe-based CLE (pCLE) with cresyl violet (CV), which has been used clinically for chromoendoscopy. We tested the feasibility of CV-assisted visualization of MP in human surgical specimens of Hirschsprung's disease, wherein the tissue dying and pCLE observation were performed from the serosal side. In the human study, rate of MP visualization by pCLE was evaluated as the primary outcome. We also evaluated the sensitivity and specificity of MP visualization by pCLE, using pathological presence/absence of MP as the gold standard. This study demonstrated the technical feasibility of visualizing the MP in real time by CV-assisted pCLE.

Free Research Field

小児外科