Newly development of analytical techniques for near infrared and raman spectroscopies using septic rat samples

2014 Fiscal Year Final Research Report

• Project/Area Number: 26670785
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Emergency medicine
• Research Institution: Kyoto University
• Principal Investigator: KOIKE KAORU 京都大学, 医学(系)研究科(研究院), 教授 (10267164)
• Co-Investigator(Kenkyū-buntansha): HIRAKAWA Keiko 日本医科大学, 医学部, 助教 (30165162) ; SUZUKI Takao 京都大学, 医学(系)研究科(研究院), 講師 (40328810) ; SATO Norio 京都大学, 医学(系)研究科(研究院), 講師 (30409205)
• Co-Investigator(Renkei-kenkyūsha): MORIYAMA Tsuyoshi 東京工芸大学, 工学部, 准教授 (80449032)
• Project Period (FY): 2014-04-01 – 2015-03-31
• Keywords: 近赤外分光法 / 敗血症 / ケモメトリクス / 複雑な混合物

Outline of Final Research Achievements

Near infrared (NIR) spectroscopy is a noninvasive, contactless and real-time analytical technology that may yield a variety of promising bedside monitoring systems in the field of emergency and critical care medicine. Rats were injected 2 mg/kg or 10mg/kg of lipopolysaccharide (LPS) intraperitoneally and the plasma was collected. The present study revealed that our technique using NIR spectra could differentiate septic plasmas obtained from LPS 2 mg/kg group and 10mg/kg group. This technique proved to be a novel signal processing method that comprehensively handles the signal of electromagnetic waves derived from complex mixture.

Free Research Field

救急医学