2015 Fiscal Year Final Research Report
Structural analysis of nucleosome containing methylated DNA
| Project/Area Number |
26840025
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Waseda University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | メチル化DNA / ペリセントロメア / ヘテロクロマチン / ヒストン / ヌクレオソーム / クロマチン |
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| Outline of Final Research Achievements |
The aim of this study is to understand the mechanism how DNA methylation affects the chromatin structure. To this end, we reconstituted the nucleosome containing methylated DNA, and performed the biochemical and structural analyses of reconstituted nucleosomes. We determined the crystal structure of the nucleosome containing methylated DNA, and found that DNA methylation does not affect the intrinsic DNA wrapping property of the nucleosome. Furthermore, we found that the DNA methylation affects the translational position of the nucleosome and the flexibility of DNA ends of the nucleosome.
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| Free Research Field |
分子生物学、生化学、構造生物学
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