Evaluation of oxidative stress in the brain of a mouse model of Alzheimer disease by in vivo EPR imaging system.

2015 Fiscal Year Final Research Report

• Project/Area Number: 26861008
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: Sapporo Medical University
• Principal Investigator: Emoto Miho 札幌医科大学, 医療人育成センター, 研究員 (10578735)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: EPRイメージング / アルツハイマー病モデルマウス / 酸化ストレス / エイジング

Outline of Final Research Achievements

Alzheimer’s disease (AD) is a neurodegenerative disease clinically characterized by progressive cognitive dysfunction. Deposition of amyloid-β (Aβ) peptides is the most important pathophysiological hallmark of AD. Oxidative stress induced by reactive oxygen species (ROS) is prominent in AD, and several reports suggest the relationship between the change in redox status and AD pathology containing progressive Aβ deposition.
electron paramagnetic resonance (EPR) imaging is used to acquire spatial information about the distribution of free radical species in vivo and visualize oxidative stress derived from ROS.
in vivo changes in redox status with age in both wild-type (WT) and AD mouse brains were measured noninvasively by three-dimensional (3D) EPR imaging using nitroxide (MCP) as a redox-sensitive probe. EPR imaging clearly visualized the accelerated change in redox status of AD mouse brain compared with WT.

Free Research Field

EPRイメージング