2016 Fiscal Year Final Research Report
Orthotopic mouse model of gastric cancer which reliably develops CTC
| Project/Area Number |
26861042
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
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| Research Institution | Nagoya University |
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Principal Investigator |
IWATA Naoki 名古屋大学, 医学部附属病院, 助教 (00719247)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | Circulating Tumor Cell / 食道癌 / 胃癌 / 大腸癌 |
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| Outline of Final Research Achievements |
Three gastric cancer cell lines (MKN1, KATO-III and MKN-45) were injected into the gastric wall of BALB/c-nu and NOD-scid mice. However, we could not find any primary tumor on gastric wall. To establish a mouse model with reproducible development of liver metastasis we used NOD-scid mice and 1x10 to the power of 6 (MKN1) were injected via portal vein. All mice developed liver metastasis. However, none of the mice had detectable Circulating Tumor Cells (CTC).
We could not established an orthotopic mouse model of gastric cancer which reliably develops CTC.
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| Free Research Field |
消化器外科学
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