Mechanism Analysis and Improvement of Fluorescence Changes of the Green Fluorescent Protein-based Citrate Sensors

2015 Fiscal Year Final Research Report

• Project/Area Number: 26870429
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biophysics; Bio-related chemistry
• Research Institution: Kyushu University
• Principal Investigator: Honda Yuki 九州大学, カーボンニュートラル・エネルギー国際研究所, 学術研究員 (90583849)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 蛍光タンパク質センサー / 蛍光クエン酸指示薬 / バイオイメージング

Outline of Final Research Achievements

The aim of this study is to elucidate the citrate-sensing mechanism and to improve sensitivity of the newly developed fluorescent protein-based sensor for citrate. Elucidation of mechanism for citrate sensing have not completed. On the other hand, applicability of the sensor to real samples was investigated for the improvement of sensitivity and the practical use.

Free Research Field

応用生物化学