Project/Area Number |
10670844
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Radiation science
|
Research Institution | Shiga University of Medical Science |
Principal Investigator |
SHO Keizen Shiga University of Medical Science, Department of Radiology, Assistant Professor, 医学部, 助手 (20283567)
|
Project Period (FY) |
1998 – 2000
|
Project Status |
Completed (Fiscal Year 2001)
|
Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
|
Keywords | nitroxide radical / L-band / electron spin resonance (ESR) / irradiation / lung / radical scavenger / ascorbic acid / 電子スピン共鳴装置 / 放射線 / 防護剤 / 放射線防護剤 / 電子スピン共鳴装置(ESR) / マウス肺 / 放射線障害 |
Research Abstract |
The change of nitroxide radical reduction capacity in the mouse lung after X-ray irradiation was investigated using a L-band electron spin resonance (ESR) spectrometer. An aqueous solution of hydroxy-2, 2, 6, 6-tetramethylpipeidine-N-oxyl (hydroxy-TEMPO) was injected through the catheter into lung of the mouse that was sacrificed by cervical spine dislocation. Rate of the nirtoxide radical reduction lineally decreased as the irradiation dose increased from 1.0 to 5.0 Gy, but no further decrease was observed at higher doses of 7.5 and 10.0 Gy. Thus it is thought that the mouse lung has two or more mechanisms of nitroxide radical reduction. One is affected by irradiation, and the other is not. Ascorbic acid administration to the mice prevented, in proportion as it's concentration increased, the decrease of nitroxide radical reduction capacity in the irradiated mouse lung, and that at 750 mg/kg or more, the capacity was higher than that of the mice which were not administered. The result suggests that ascorbic acid is effective in preventing from the radiation injury of the lung after radiation therapy.
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