The development of teaching materials to show visually DNA as the 'color-code' genome, entire chromosomes or any of their parts using in situ hybridization technique.
| Project/Area Number |
13680201
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Science education
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| Research Institution | Tottori University |
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Principal Investigator |
TAKAHASHI Chigusa Tottori University, Faculty of Education and Regional Sciences, Associate Professor, 教育地域科学部, 助教授 (00226846)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2003: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | DNA / development of teaching material / genome / in situ hybridization / gamete / reductional division / chromosome recombination / 交叉・組み換え / 遺伝子DNAの教材化 / 染色体ペインティング / 体細胞分裂 / 減数分裂 / in situ ハイブリダイゼーション |
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| Research Abstract |
1.The development of teaching materials to show visually the two parental genomes in the F1 hybrid using genomic in situ hybridization (GISH).
GISH was clearly able to distinguish between the two parental genomes in the bigeneric F1 hybrid Gasteria lutzii x Aloe aristata, when genomic DNA of either G.lutzii or A.aristata was used as a probe. The Gasteria genome (four long, three short chromosomes) were fluorescent yellow ; the Aloe genome (four long, three short chromosomes ) fluorescent orange. Using the GISH results, teaching materials to show visually two different genome from different parents in the F1 hybrid were developed.
2.The development of teaching materials to show visually meiotic pairing, separation and recombination of chromosomes by genomic in situ hybridization (GISH).
Teaching materials to show visually meiotic pairing, separation and recombination of chromosomes in a bigeneric hybrid Gasteria lutzii x Aloe aristata were developed by genomic in situ hybridization (GISH) using the DNA of the original species as a probe. Chromosome painting by GISH enabled the direct and clear study of homoeologous chromosome behavior during the meiotic process. That is, it become possible through color-coding to present visually the following : reduction of chromosome numbers by the bivalent formation and random assortment of paired chromosomes and segregation to both poles at first meiotic division, followed by a separation of chromatids of each chromosome to the opposite poles and transmission of them to the gametes at second meiotic division.
Using the pictures provided by GISH, students could understand the mechanism of separation and recombination of chromosomes as different genomes. Then, students could acknowledge that intergenomic recombinations were incorporated into the gametes.
3.The development of efficient and reliable protocols, which include a detailed practical guide for student use.
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Report
(4 results)
Research Products
(13 results)
