Development of a visualization method by using HCR-FISH and Click Chemistry
| Project/Area Number |
15H06814
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Civil and environmental engineering
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| Research Institution | Matsue National College of Technology |
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Principal Investigator |
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| Project Period (FY) |
2015-08-28 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 高感度FISH法 / HCR法 / click chemistry / Click chemistry / FISH法 / 環境微生物 / 微生物 / 視覚的検出 / 原核生物 |
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| Outline of Final Research Achievements |
In this study, combination with in situ DNA-hybridization chain reaction (HCR) and click chemistry was developed for successfully detecting microorganisms in oligotrophic. Although It high specificity by using Escherichia coli was revealed, click chemistry was not successful. It may be applicable for detection of an environmental microorganism after optimization of click chemistry in situ.
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Report
(3 results)
Research Products
(9 results)
