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Study on a novel phenomenon of horizontal gene transfer in bacterial colony biofilms

Research Project

Project/Area Number 19580089
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionNara Women's University

Principal Investigator

MAEDA Sumio  Nara Women's University, 生活環境学部, 准教授 (90335472)

Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords微生物生態学 / 遺伝子水平伝播 / バイオフィルム / 大腸菌 / 形質転換 / 枯草菌
Research Abstract

This study aimed at revealing the detail and the generality of nonconjugative, nonviral horizontal gene transfer in colony biofilms of Escherichia coli. We found that this horizontal gene transfer is a novel kind of transformation, that there are several dozens of genes which are involved in this gene transfer in E.coli, and that this gene transfer occurs on the culture on food-based media as well as between E.coli and Bacillus subtilis.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (22 results)

All 2009 2008 2007 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (14 results) Remarks (2 results)

  • [Journal Article] Horizontal transfer of non-conjugative plasmid in colony biofilm of Escherichia coli on food-based media2009

    • Author(s)
      Tsuyako Ando, Sumio Maeda, (ほか3名、5番目)
    • Journal Title

      World Journal of Microbiology and Biotechnology 25

      Pages: 1865-1869

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Horizontal transfer of non-conjugative plasmid in colony biofilm of Escherichia coli on food-based media.2009

    • Author(s)
      Tsuyako Ando, Sumio Maeda, et al.
    • Journal Title

      World Journal of Microbiology and Biotechnology 25

      Pages: 1865-1869

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Freeze-thaw-induced lateral transfer of non-conjugative plasmids by in situ transformation in Escherichia coli in natural waters and food extracts2008

    • Author(s)
      Yuko Ishimoto, Shiho Kato, Sumio Maeda
    • Journal Title

      World Journal of Microbiology and Biotechnology 24

      Pages: 2731-2735

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Freeze-thaw-induced lateral transfer of non-conjugative plasmids by in situ transformation in Escherichia coli innatural waters and food extracts.2008

    • Author(s)
      Yuko Ishimoto, Sumio Maed a et. al.
    • Journal Title

      World Journal of Microbiology andbiotechnology 24

      Pages: 2731-2735

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] バイオフィルム状微生物の遺伝子転移に関する研究2008

    • Author(s)
      前田 純夫
    • Journal Title

      IFO Research Communications 21

      Pages: 71-81

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] バイオフィルム状微生物の遺伝子転移に関する研究2007

    • Author(s)
      前田純夫
    • Journal Title

      IFO Research Communications 21

      Pages: 71-81

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Presentation] 大腸菌のcell-to-cell transformationに抑制的に関与する遺伝子の網羅的スクリーニング2009

    • Author(s)
      松田彩子、前田純夫, (ほか10名、12番目)
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-12
    • Related Report
      2009 Final Research Report
  • [Presentation] 大腸菌のcell-to-cell transformationに必須あるいは促進的に関与する遺伝子の網羅的スクリーニング2009

    • Author(s)
      黒野尚美、前田純夫, (ほか6名、8番目)
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-12
    • Related Report
      2009 Final Research Report
  • [Presentation] 大腸菌のcel1-to-cell transformationに必須あるいは促進的に関与する遺伝子の網羅的スクリーニング2009

    • Author(s)
      黒野尚美、前田純夫, ら
    • Organizer
      第32回分子生物学会年
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] 大腸菌のcell-to-cell transformationに抑制的に関与する遺伝子の網羅的スクリーニング2009

    • Author(s)
      松田彩子、前田純夫, ら
    • Organizer
      第32回分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] 大腸菌コロニーバイオフィルム中での非接合性プラスミド水平伝播に対する特定プラスミド配列の影響2008

    • Author(s)
      祖父江里奈、前田純夫, (ほか3名、5番目)
    • Organizer
      第31回日本分子生物学会・第81回日本生化学会合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2008-12-12
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] 大腸菌間の非接合性プラスミド水平伝播機構の解析2008

    • Author(s)
      越中屋里香、前田純夫, (ほか3名、5番目)
    • Organizer
      第31回日本分子生物学会・第81回日本生化学会合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2008-12-12
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] Horizontal plasmid transfer in a bacterial colony biofilm2008

    • Author(s)
      Rina Sobue, Sumio Maeda, (ほか4名、6番目)
    • Organizer
      IUMS Congress 2 008, XII. International Congress of Bacterio logy and Applied Microbiology
    • Place of Presentation
      Istanbul Convention Center, Istanbu l, Turkey
    • Year and Date
      2008-08-09
    • Related Report
      2009 Final Research Report
  • [Presentation] Horizontal transfer of nonco njugative plasmids in a colony biofilm of E. coli.2008

    • Author(s)
      Rika Etchuuya, Sumio Maeda, (ほか4名、6番目)
    • Organizer
      IUMS Congress 2008, XII. Interna tional Congress of Bacteriology and Applie d Microbiology
    • Place of Presentation
      Istanbul Convention Center, Istanbul, Turkey
    • Year and Date
      2008-08-09
    • Related Report
      2009 Final Research Report
  • [Presentation] Horizontal transfer of nonconjugative plasmids in a colony biofilm of E. coli.2008

    • Author(s)
      Rika Etchuya, Sumio Maedaet. al.
    • Organizer
      IUMS Congress 2008, XII. International Congressof Bacteriology and Applied Microbiology
    • Place of Presentation
      Istanbul Convention Center, Istanbul, Turkey
    • Year and Date
      2008-08-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] Horizontal plasmid transfer in a bacterial colony biofilm.2008

    • Author(s)
      Rina Sobue, Sumio Maeda et. al.
    • Organizer
      IUMS Congress 2008, XII. International Congressof Bacteriology and Applied Microbiology
    • Place of Presentation
      Istanbul Convention Center, Istanbul, Turkey
    • Year and Date
      2008-08-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] 細菌コロニーバイオフィルムにおけるプラスミド水平伝播の解析2007

    • Author(s)
      祖父江里奈、前田純夫, (ほか4名、6番目)
    • Organizer
      第30回日本分子生物学会・第80回日本生化学会合同大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-12-11
    • Related Report
      2009 Final Research Report
  • [Presentation] 大腸菌コロニーバイオフィルムにおける非接合性プラスミド水平伝播機構の解析2007

    • Author(s)
      越中屋里香、前田純夫, (ほか4名、6番目)
    • Organizer
      第30回日本分子生物学会・第80回日本生化学会合同大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-12-11
    • Related Report
      2009 Final Research Report
  • [Presentation] 大腸菌コロニーバイオフィルムにおける非接合性プラスミド水平伝播機構の解析2007

    • Author(s)
      越中屋里香
    • Organizer
      日本分子生物学会・日本生化学会合同大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-12-11
    • Related Report
      2007 Annual Research Report
  • [Presentation] 細菌コロニーバイオフィルムにおけるプラスミド水平伝播の解析2007

    • Author(s)
      祖父江里奈
    • Organizer
      日本分子生物学会・日本生化学会合同大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-12-11
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://www.nara-wu.ac.jp/life/food/foodsafe/maeda/

    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.nara-wu.ac.jp/life/food/foodsafe/maeda/

    • Related Report
      2009 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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