Biological effect of Heavy ion exposure from horizontal and vertical directions.

• Project/Area Number: 19710056
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: National Institute of Radiological Sciences
• Principal Investigator: KATO Takamitsu National Institute of Radiological Sciences, 重粒子医科学センター, 研究員 (30450430)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,600,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥2,300,000 (Direct Cost: ¥2,300,000)
• Keywords: 放射線生物影響 / 放射線 / DNA損傷 / DNA修復 / 重粒子線 / NDA二本鎖切断

Research Abstract

接着培養細胞に対して、粒子放射線を水平方向、および垂直方向から照射し、その入射角によって、生物効果がどのように変わるのかを研究した。水平方向照射の特徴は、入射トラック数は少ないが、一粒子当りのDNA損傷は多く、垂直方向照射はその逆である。細胞致死効果、染色体異常ともに、水平方向に比べ、垂直方向照射が効率的であり、トラック間の相互作用によって生物効果に差があることが示唆された。

Research Products

• [Journal Article] Signatures of DNA double strand breaks produced in irradiated G1 and G2 cells persist into mitosis (2009)
  • Author(s): Kato TA, Okayasu R, Bedford JS
  • Journal Title: J Cell Physiol. Jun 219(3) Pages: 760-5
  • Peer Reviewed
• [Journal Article] Signatures of DNA double strand breaks produced in irradiated G1 and G2 cells persist into mitosis. (2009)
  • Author(s): Kato TA, et al.
  • Journal Title: Journal of Cellular Physiology 219 Pages: 760-765
  • Peer Reviewed
• [Journal Article] Ionizing radiation downregulates ASPM, a gene responsible for microcephaly in humans (2008)
  • Author(s): Fujimori A, Yaoi T, Ogi H, Wang B, Suetomi K, Sekine E, Yu D, Kato T, Takahashi S, Okayasu R, Itoh K, Fushiki S
  • Journal Title: Biochem Biophys Res Commun. May 9 Epub 2008 369(3) Pages: 953-7
  • Peer Reviewed
• [Journal Article] Comparison of the Induction and Disappearance of DNA Double Strand Breaks and γ-H2AX Foci after Irradiation of Chromosomes in G1-phase or in Condensed Metaphase Cells (2008)
  • Author(s): Kato TA, Okayasu R, Bedford JS
  • Journal Title: Mutation Research: Molecular and Fundamental Mechanisms Mar 1 Epub 2007 639(1-2) Pages: 108-12
  • Peer Reviewed
• [Journal Article] Comparison of the induction and disappearance of DNA double strand breaks and gamma-H2AX foci after irradiation of chromosomes in G1-phase or in condensed metaphase cells. (2008)
  • Author(s): Kato TA, et al.
  • Journal Title: Mutation Research 639 Pages: 108-12
  • Peer Reviewed
• [Journal Article] Ionizing radiation downregulates ASPM, a gene responsible for microcephaly in humans. (2008)
  • Author(s): Fujimori A, et al.
  • Journal Title: Biochemistry Biophysics Research Communication 369 Pages: 953-957
  • Peer Reviewed
• [Journal Article] Comparison of the induction and disappearance of DNA double strand breaks and gamma-H2AX foci after irradiation of chromosomein Gl-phase or in condensed metaphase cells (2008)
  • Author(s): Kato TA, Okayasu R, Bedford JS.
  • Journal Title: Mutation Research 639 Pages: 108-112
  • Peer Reviewed
• [Journal Article] Cytotoxicity of cigarette smoke condensate is not due to DNA double strand breaks: Comparative studies using radiosensitive mutant and wild-type CHO cells (2007)
  • Author(s): Kato T, Nagasawa H, Warner C, Okayasu R, Bedford JS
  • Journal Title: International Journal of Radiation Biology. Sep 83(9) Pages: 583-91
  • Peer Reviewed
• [Journal Article] A defect in DNA double strand break processing in cells from unaffected parents of retinoblastoma patients and other apparently normal humans (2007)
  • Author(s): Kato TA, Wilson PF, Nagasawa H, Fitzek MM, Weil MM, Little JB, Bedford JS
  • Journal Title: DNA Repair (Amst). Jun 1Epub 2007 6(6) Pages: 818-29
  • Peer Reviewed
• [Presentation] DNA損傷修復欠損株における重粒子線照射に対する細胞周期の影響 (2008)
  • Author(s): 加藤宝光
  • Organizer: 放射線影響学会
  • Place of Presentation: 小倉
  • Year and Date: 2008-11-20
• [Presentation] Cell Cycle Effect for DNA repair deficient CHO mutant cells irradiated by Heavy Charged Particles (2008)
  • Author(s): Kato TA
  • Organizer: 放射線影響学会
  • Place of Presentation: 小倉
• [Presentation] The Massive Radiation Exposure and its Risk Management (2008)
  • Author(s): Kato TA
  • Organizer: International Crisis Management Symposium on CBRN and Emerging Infectious Diseases
  • Place of Presentation: Choshi, Chiba, Japan
• [Presentation] Heavy ion irradiation affects two major repair pathways for DNA double strand breaks (2008)
  • Author(s): 加藤宝光
  • Organizer: Radiation Research Society Meeting
  • Place of Presentation: USA, Boston
• [Presentation] DNA Repair in Heavy Ion Irradiated Cells Low & High LET/Cel Cycle (2007)
  • Author(s): Kato TA
  • Organizer: Tolmach Symposium on"Chromatin Structure and DNA Repair"at Washington University, St Louis
  • Place of Presentation: St. Louis, USA
  • Year and Date: 2007-12-01
• [Presentation] Radiosensitivity of Heavy Charged Particles Irradiated various DNA repair deficient CHO mutant cells (2007)
  • Author(s): Kato TA, Fujii Y, Seo K, Bedford JS, Thompson JH, Fujimori A, Okayasu R
  • Organizer: 放射線影響学会
  • Place of Presentation: 幕張、千葉
  • Year and Date: 2007-11-16
• [Presentation] DNA Repair in Heavy Ion Irradiated Cells Low & High LET/Cell Cycle (2007)
  • Author(s): Kato TA
  • Organizer: Tolmach Symposium on "Chromatin Structure and DNA Repair"
  • Place of Presentation: St Louis at Washington University
• [Presentation] The Effect of Heavy Charged Particles on Synchronously Dividing CHO cells (2007)
  • Author(s): Kato TA
  • Organizer: International Congress of Radiation Research
  • Place of Presentation: San Francisco
• [Presentation] Radiosensitivity of Heavy Charged Particles Irradiated various DNA repair deficient CHO mutant cells (2007)
  • Author(s): Kato TA
  • Organizer: 放射線影響学会
  • Place of Presentation: 千葉