Intensive mapping of diplospory gene locus and exploitation of candidate gene for diplospory in Chinese chive

• Project/Area Number: 21780030
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Horticulture/Landscape architecture
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: YAMASHITA Ken-ichiro 独立行政法人農業・食品産業技術総合研究機構, 野菜茶業研究所・野菜育種・ゲノム研究領域, 主任研究員 (80414671)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: アポミクシス / アリウム / 単為発生性 / ニラ / 複相大胞子形成性 / 複相大胞子形成 / 単為発生 / Allium / トリソミック / DNAマーカー / ニフ

Research Abstract

Genetic maps of diplospory(D) and parthenogenesis(P) gene loci were constructed by linkage analysis with AFLP, RAPD and ISSR markers in the heteroploid population(BC1) in Chinese chive. Degrees of diplospory and parthenogenesis were evaluated in the diploid-hyperdiploid population(BC2) produced by the cross between amphimictic diploid and apomictic hypotriploid BC1 plants. As the results, four plants with low diplospory values and one plant with relatively high parthenogenesis value were identified. By the Feulgen staining of root tip cells in the parthenogenetic plant, it was revealed that this plant was a trisomic(2n=17, 2x+1), which indicates that the P locus is located on the additional chromosome.

Research Products

• [Journal Article] Modes of Inheritance of two apomixis components, diplospory and parthenogenesis, in Chinese chive(Allium ramosum) revealed by analysis of the segregating population generated by back-crossing between amphimictic and apomictic diploids (2012)
  • Author(s): Yamashita K., Y. Nakazawa, K. Namai, M. Amagai, H. Tsukazaki, T. Wako, A. Kojima
  • Journal Title: Breeding Science Volume: 62 Pages: 160-169
  • NAID: 10030434650
  • Peer Reviewed
• [Journal Article] Modes of inheritance of two apomixis components, diplospory and parthenogenesis, in Chinese chive (Allium ramosum) revealed by analysis of the segregating population generated by back-crossing between amphimictic and apomictic diploids
  • Author(s): 山下謙一郎
  • Journal Title: Breeding Science Volume: (掲載確定)(未定)
  • NAID: 10030434650
  • Peer Reviewed
• [Presentation] Modes of Inheritance of Two Apomixis Components, Diplospory and Parthenogenesis, in Chinese chive(Allium ramosum) (2012)
  • Author(s): Yamashita, K., Y. Nakazawa, K. Namai, M. Amagai, H. Tsukazaki, T. Wako, A. Kojima
  • Organizer: 6th International Symposium on Edible Alliaceae
  • Place of Presentation: アクロス福岡(福岡県)
  • Year and Date: 2012-05-22
• [Presentation] ニラ両性生殖性二倍体×アポミクシス性低三倍体による単為発生性トリソミックの作出 (2012)
  • Author(s): 山下謙一郎、塚崎光、小島昭夫、谷口成紀、若生忠幸
  • Organizer: 園芸学会平成24年度春季大会
  • Place of Presentation: 大阪府立大学中百舌鳥キャンパス(大阪府)
  • Year and Date: 2012-03-28
• [Presentation] ニラ異数性分離集団を用いた複相大胞子形成遺伝子座および単為発生遺伝子座のマッピング (2011)
  • Author(s): 山下謙一郎、塚崎光、若生忠幸、小島昭夫
  • Organizer: 園芸学会平成23年度春季大会
  • Place of Presentation: 宇都宮大学農学部(栃木県)
  • Year and Date: 2011-03-21
• [Presentation] ニラ両性生殖性二倍体×アポミクシス性低三倍体による正二倍体・高二倍体分離集団の育成 (2010)
  • Author(s): 山下謙一郎、塚崎光、小島昭夫、若生忠幸
  • Organizer: 園芸学会平成22年度春季大会
  • Place of Presentation: 日本大学生物資源科学部(神奈川県)
  • Year and Date: 2010-03-22
• [Presentation] ニラの複相大胞子形成性に連鎖するDNAマーカーの開発 (2009)
  • Author(s): 山下謙一郎、塚崎光、若生忠幸、小島昭夫
  • Organizer: 園芸学会平成21年度秋季大会
  • Place of Presentation: 秋田大学手形キャンパス(秋田県)
  • Year and Date: 2009-09-27