Intensive mapping of diplospory gene locus and exploitation of candidate gene for diplospory in Chinese chive
| Project/Area Number |
21780030
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Horticulture/Landscape architecture
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
YAMASHITA Ken-ichiro 独立行政法人農業・食品産業技術総合研究機構, 野菜茶業研究所・野菜育種・ゲノム研究領域, 主任研究員 (80414671)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | アポミクシス / アリウム / 単為発生性 / ニラ / 複相大胞子形成性 / 複相大胞子形成 / 単為発生 / Allium / トリソミック / DNAマーカー / ニフ |
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| Research Abstract |
Genetic maps of diplospory(D) and parthenogenesis(P) gene loci were constructed by linkage analysis with AFLP, RAPD and ISSR markers in the heteroploid population(BC1) in Chinese chive. Degrees of diplospory and parthenogenesis were evaluated in the diploid-hyperdiploid population(BC2) produced by the cross between amphimictic diploid and apomictic hypotriploid BC1 plants. As the results, four plants with low diplospory values and one plant with relatively high parthenogenesis value were identified. By the Feulgen staining of root tip cells in the parthenogenetic plant, it was revealed that this plant was a trisomic(2n=17, 2x+1), which indicates that the P locus is located on the additional chromosome.
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Report
(4 results)
Research Products
(7 results)
