| Project/Area Number |
21780090
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Applied biochemistry
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
IWAMOTO Taku The University of Tokyo, 大学院・農学生命科学研究科, リサーチフェロー (00401191)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
|---|
| Keywords | 細胞応答 / 腸管上皮細胞 / 細胞分化 / 免疫学 / Toll様受容体 / サイトカイン / 小腸 / 大腸 / 食品 |
|---|
| Research Abstract |
The intestines are constantly exposed to commensal and pathogenic microbes and dietary substances including food antigens ; therefore, intestines have a unique immune system to fight against or to cope with them. Intestinal epithelial cells (IECs), which are located at the surface of intestinal mucosa, play an important role in regulating the intestinal immune system. Thus it would appear that it is important for analysis of the effects of immunity-modulating drugs and functional foods on immune function of IECs. In this work, we try to establish and analyze of immune functions of intestinal epithelial cell lines. We successfully established mouse epithelial cell lines from fetal and adult mouse small and large intestine. Expression pattern of some immune regulatory molecules in small and large intestinal epithelium corresponds to those in small and large IEC lines respectively. We have also found using these IEC lines that treatment with sphingosine-1-phosphate regulates the mRNA expression and secretion of some cytokines and chemokines. These in vitro model systems may prove useful for detailed analysis of the immune regulatory functions of the small and large intestinal epithelia, and for screening immunity-modulating drugs and functional foods.
|
