The molecular mechanism of catch contraction based on the structural characteristics of twitchin of bivalve smooth muscle
| Project/Area Number |
21780195
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Fisheries chemistry
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| Research Institution | Mie University |
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Principal Investigator |
FUNABARA Daisuke Mie University, 大学院・生物資源学研究科, 准教授 (00335150)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | キャッチ収縮 / 二枚貝 / ミオシン / アクチン / トゥイッチン / リン酸化 / 等温滴定カロリメトリー / RNA干渉 |
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| Research Abstract |
Myosin S1 of catch muscle of mussel Mytilus galloprovincialis was expressed using a protein expression system using the wheat germ extract. The myosin binding regions on the twitchin molecule were predicted with the ITC analysis using the peptides designed according to the sequence of twitchin and the myosin loop 2 peptide. The expression of myosin and paramyosin genes in catch muscles of mussel was suppressed by the RNA interference methods.
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Report
(3 results)
Research Products
(8 results)
