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Analysis of the uptake mechanism for double stranded RNA

Research Project

Project/Area Number 21790457
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Immunology
Research InstitutionHokkaido University

Principal Investigator

WATANABE Ayako  Hokkaido University, 大学院・医学研究科, 博士研究員 (00533423)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords二重鎖RNA / 膜受容体 / Toll-like receptor 3 / Toll-like receptor3
Research Abstract

we show that the cytoplasmic lipid raft protein, Raftlin, is essential for poly(I:C) cellular uptake in human myeloid DCs and epithelial cells. When Raftlin was silenced, poly(I:C) failed to enter cells and induction of IFN-beta production was inhibited. In addition, cellular uptake of B-type oligodeoxynucleotide that shares its uptake receptor with poly(I:C) was suppressed in Raftlin knockdown cells. Upon poly(I:C) stimulation, Raftlin was translocated from the cytoplasm to the plasma membrane where it colocalized with poly(I:C), and thereafter moved to TLR3-positive endosomes. Thus, Raftlin cooperates with the uptake receptor to mediate cell entry of poly(I:C), which is critical for activation of TLR3.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (7 results)

All 2011 2010 2009

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (4 results)

  • [Journal Article] RAFTLIN IS INVOLVED IN THE NUCLEOCAPTURE COMPLEX TO INDUCE POLY(I:C)-MEDIATED TLR3 ACTIVATION.2011

    • Author(s)
      Ayako Watanabe, Megumi Tatematsu, Kazuko Saeki, Sachiko Shibata, Hiroaki Shime, Akihiko Yoshimura, Chikashi Obuse, Tsukasa Seya, Misako Matsumoto
    • Journal Title

      J.Biol.Chem. 286

      Pages: 10702-10711

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Raftlin is involved in the nucleocapture complex to induce poly(I:C)-mediated TLR3 activation.2011

    • Author(s)
      渡部綾子, 松本美佐子, ら
    • Journal Title

      J.Bilo.Chem.

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hepatitis C virus core protein abrogates the DDX3 function that enhances IPS-1-mediated IFN-beta induction.2010

    • Author(s)
      Oshiumi.H., M.Ikeda, M.Matsumoto, A.Watanabe, O.Takeuchi, S.Akira, N.Kato, K.Shimotohno, T.Seya.
    • Journal Title

      Plos ONE 5(12)

    • NAID

      120002697703

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] Identification of a novel protein that participates in poly(I:C) cellular uptake2010

    • Author(s)
      渡部綾子
    • Organizer
      第14回 国際免疫学会議
    • Place of Presentation
      神戸ポートビアホテル、神戸国際展示場
    • Year and Date
      2010-08-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] Identification of a novel protein that participates in poly (I:C) cellular uptake.2010

    • Author(s)
      Watanabe A., Seya T., Matsumoto M.
    • Organizer
      14^<th> International Congress of Immunology.(Workshop)
    • Place of Presentation
      Kobe
    • Related Report
      2010 Final Research Report
  • [Presentation] 二重鎖RNA取り込みに関わるタンパク質の解析2009

    • Author(s)
      渡部綾子
    • Organizer
      日本免疫学会
    • Place of Presentation
      大阪国際会議場(大阪)
    • Year and Date
      2009-12-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] Analysisof the uptake protein for double-stranded RNA2009

    • Author(s)
      渡部綾子、瀬谷司、松本美佐子
    • Organizer
      第39回日本免疫学会総会・学術集会(口頭)
    • Place of Presentation
      大阪
    • Related Report
      2010 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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