Toxicogenomic analysis of pyrroloquinoline quinone of vitamin-like compound

Research Project

Project/Area Number	21790550
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Hygiene
Research Institution	University of the Ryukyus
Principal Investigator	TACHIBANA Shinjiro 琉球大学, 農学部, 准教授 (70447655)
Project Period (FY)	2009 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000) Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords	ピロロキノリンキノン / ビタミン / マイクロアレイ / トキシコゲノミクス / 骨粗鬆症 / 骨芽細胞
Research Abstract	In this study, we analyzed cytotoxicity and gene expression during osteogenesis by addition of pyrroloquinoline quinone(PQQ) showing vitamin-like bioactivity on murine osteoblast. The osteoblast treated with PQQ showed cytotoxicity and growth inhibition at the concentration of 0. 1 mM. During osteogenesis after differentiation, the presence of PQQ increases the enzyme activity of alkaline phosphatase known as the marker enzyme of the osteogenesis, although the osteoblast exhibited the decreasing of mineralization along with the concentration-dependent of PQQ.