| Project/Area Number |
21790691
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Kurume University |
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Principal Investigator |
KUWAHARA Reiichiro Kurume University, 医学部, 助教 (30389275)
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| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 肝臓病学 / 肝幹細胞 / 上皮間葉転換 / 肝細胞ニッチェ / 臓器再生 |
|---|
| Research Abstract |
【Animal experiment】 Protocols for double-immunostaining of hepatic stem/progenitor cell marker (pan keratin) and EMT regulators (Snail, SIP 1, Twist) were established in murine liver tissue. Analyses in the murine liver, leathery injured by APAP, revealed that mesenchymal marker (S100A4) were expressed in the oval cells after administration of APAP.
【Human liver】 For the analyses of EMT in hepatic stem/progenitor cell of human liver, protocols for double-immunostaining of hepatic stem/progenitor cell marker (EpCAM), epithelial markers (keratin 19 and E-cadherin), EMT regulators (Snail, SIP 1, Twist) and mesenchymal marker (S100A4) were established in human liver tissue. Explanted livers for liver transplantation were obtained from patients and analyzed by immunostaining methods. These analyses highlighted that EMT regulators (Snail and Twist) were expressed in the epithelial ductular cells when liver was leathery injured.
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