Functional analysis of cochlear outer hair cells in a dominant-negative connexin26 mutant mouse.
| Project/Area Number |
21791648
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Juntendo University |
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Principal Investigator |
NARUI Yuya Juntendo University, 医学部, 助教 (60453578)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | gjb2遺伝子 / 優性阻害効果 / マウス変異体 / 歪成分耳音響放射 / 外有毛細胞 / gib2遺伝子 / 内耳 / GJB2 / ギャップジャンクション |
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| Research Abstract |
Distortion product otoacoustic emissions (DPOAEs) have been used to examine the development of hearing in the rat and gerbil. However, no reports of DPOAE measurement from the onset of hearing in mice are available. Commercially-available components were assembled and adapted to provide a suitable probe microphone and sound delivery system for measuring DPOAE in developing C57BL/6J mice. Furthermore, DPOAE data were compared with the findings of the auditory brainstem response (ABR). DPOAEs were obtained at 8 kHz from 11 days after birth, 20 kHz from 12 days, and 30 kHz from 13 days. Adult-like patterns of DPOAE were obtained 21 days at 8 and 20 kHz, and 28 days at 30 kHz. On the other hand, the ABR thresholds at 12 to 36 kHz appeared between 11 and 12 days and were saturated at 14 days. Based on these data, the onset of measureable DPOAEs in the mouse were earlier than in the rat and gerbil. The maturation of DPOAE in the mouse begins at a lower frequency in the high frequency range. In addition, the ABR threshold reached maturation earlier than DPOAE.
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Report
(3 results)
Research Products
(3 results)
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[Journal Article] Cochlear outer hair cells in a dominant-negative connexin26 mutant mouse preserve non-linear capacitance in spite of impaired distortion product otoacoustic emission.2009
Author(s)
Minekawa A, Abe T, Inoshita A, Iizuka T, Kakehata S, Narui Y, Koike T, Kamiya K, Okamura HO, Shinkawa H, Ikeda K.
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Journal Title
Neuroscience 164
Pages: 1312-1319
Related Report
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