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Elucidating signaling components under low potassium conditions

Research Project

Project/Area Number 21870045
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Plant molecular biology/Plant physiology
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

SHIN Ryoung  The Institute of Physical and Chemical Research, 機能調節研究ユニット, ユニットリーダー (80525577)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥2,756,000 (Direct Cost: ¥2,120,000、Indirect Cost: ¥636,000)
Fiscal Year 2010: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000)
Fiscal Year 2009: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
Keywordsカリウムトランスポーター / FOXライブラリ / 栄養欠乏 / 非生物的ストレス / 転写因子
Research Abstract

Arabidopsis FOX (Full-length cDNA over-expressor genes) agrobacterium librarys were transformed into established AtHAK5 promoter::luciferase Arabidopsis plants. These AtHAK5 promoter::luciferase plants with FOX library have been screened and more than 200 candidate transgenic plants that have higher luciferase activity under full nutrient condition have been isolated. Among them, fifteen lines were analyzed to determine which gene is overexpressed, and some candidates were analyzed for their expression in response to nutrient deficiency. The overexpression plants and knockout plants of WRKY TF were obtained. The overexpression plants showed increased tolerance to potassium deficient condition.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (7 results)

All 2010 2009 Other

All Presentation (6 results) Remarks (1 results)

  • [Presentation] Dissection of Potassium Signaling in Arabidopsis thaliana2010

    • Author(s)
      申怜
    • Organizer
      21^<st> International Conference on Arabidopsis Research
    • Place of Presentation
      Yokohama, Japan
    • Related Report
      2010 Final Research Report
  • [Presentation] Dissection of Potassium Signaling in Arabidopsis Thaliana2010

    • Author(s)
      Ryoung Shin
    • Organizer
      21^<st> International Conference on Arabidopsis Research
    • Place of Presentation
      横浜
    • Related Report
      2010 Annual Research Report
  • [Presentation] Identification of Arabidopsis potassium deficient signaling components via full-length cDNA overexpressor (FOX) gene hunting system and AtHAK52009

    • Author(s)
      申怜
    • Organizer
      Plant Genomics and Beyond
    • Place of Presentation
      Evry, France
    • Year and Date
      2009-07-06
    • Related Report
      2010 Final Research Report
  • [Presentation] Identification of Arabidopsis potassium deficient signaling components via full-length cDNA overexpressor(FOX)gene hunting system and AtHAK52009

    • Author(s)
      申怜
    • Organizer
      Plant Genomics and Beyond
    • Place of Presentation
      Evry, France
    • Year and Date
      2009-07-06
    • Related Report
      2009 Annual Research Report
  • [Presentation] Identification of Arabidopsis potassium deficient signaling components via full-length cDNA overexpressor (FOX) gene hunting system and AtHAK52009

    • Author(s)
      申怜
    • Organizer
      20^<th> International Conference on Arabidopsis Research
    • Place of Presentation
      Edinburgh, Scotland
    • Year and Date
      2009-07-02
    • Related Report
      2010 Final Research Report
  • [Presentation] Identification of Arabidopsis potassium deficient signaling components via full-length cDNA overexpressor(FOX)gene hunting system and AtHAK52009

    • Author(s)
      申怜
    • Organizer
      International Conference on Arabidopsis Research
    • Place of Presentation
      Edinburgh, Scotland
    • Year and Date
      2009-07-02
    • Related Report
      2009 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://labs.psc.riken.jp/rnru/index.html

    • Related Report
      2010 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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