Screening of an L-amino acid ligase that recognizes acidic amino acids as N-terminal substrates and its application
Project/Area Number |
21880045
|
Research Category |
Grant-in-Aid for Research Activity Start-up
|
Allocation Type | Single-year Grants |
Research Field |
Applied biochemistry
|
Research Institution | Waseda University |
Principal Investigator |
ARAI Toshinobu Waseda University, 理工学術院, 助手 (00547622)
|
Project Period (FY) |
2009 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥2,756,000 (Direct Cost: ¥2,120,000、Indirect Cost: ¥636,000)
Fiscal Year 2010: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000)
Fiscal Year 2009: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
|
Keywords | L-アミノ酸リガーゼ / ペプチド合成 / 抗生物質 / L-amino acid ligase / peptide synthesis / plumbemycin |
Research Abstract |
We searched for a novel L-amino acid ligase (Lal) possessing the activity toward acidic amino acids from a microorganism producing peptide antibiotics. A novel ligase that seemed to be involved in the biosynthesis of the antibiotics was obtained by enzyme purification and gene manipulation, but the search of the Lal showing the activity toward acidic amino acids has been under examination.
|
Report
(3 results)
Research Products
(5 results)