| Project/Area Number |
24860030
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Niigata University |
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Principal Investigator |
ISHIYAMA Yohei 新潟大学, 産学地域連携推進機構, 研究員 (50634854)
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 抗菌ペプチド / 抗生物質 / 無細胞タンパク質合成 / 微生物 / 作用機構 / タンパク質合成 |
|---|
| Research Abstract |
The purpose of this study was to reveal modes of action of antimicrobial peptides in cells. Several methods using cell-free protein synthesis system were developed for achieving this purpose. By using our methods, we could confirm the known modes of action of conventional antibiotics, such as streptomycin, fosfomycin and BLEOCIN. We were also able to not only assess the unknown abilities of antimicrobial peptides to inhibit protein synthesis, but also elucidate the inhibitory steps in protein synthesis process, including transcription, translation and refolding. Moreover, we found that a purification method using antimicrobial peptide-immobilized magnetic beads was effective to isolate target compounds in cells.
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