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Molecular basis of immune receptor conferring dual resistance to virus and oomycete and its application for disease control

Research Project

Project/Area Number 26292022
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Plant protection science
Research InstitutionTohoku University

Principal Investigator

Takahashi Hideki  東北大学, 農学研究科, 教授 (20197164)

Co-Investigator(Kenkyū-buntansha) 安藤 杉尋  東北大学, 農学研究科, 准教授 (10442831)
Project Period (FY) 2014-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Keywordsウイルス / NB-LRRクラス抵抗性タンパク質 / エピジェネティクス / 病害抵抗性 / NB-LRRタンパク質 / ウィルス / 植物保護 / NB-LRR class Rタンパク質 / 植物病理 / ウイルス抵抗性 / キュウリモザイクウイルス / 病害抵抗性遺伝子
Outline of Final Research Achievements

RCY1/HRT/RPP8 locus of Arabidopsis thaliana encodes NB-LRR class R-protein conferring the resistance to CMV, TCV and Hpa. The expression of RCY1/HRT/RPP8 locus was regulated by epigenetic modification through cytosine methylation of its promoter region, and accumulated level of R-protein was correlated with degree of disease resistance to pathogens. RCY1/HRT/RPP8 R-protein can recognize the molecular structure of multiple complex of CP mediated by N-terminal α-helix domain of CP.

Academic Significance and Societal Importance of the Research Achievements

(1)抵抗性対立遺伝子座RCY1、HRT、RPP8は、エピジェネティックな調節により発現制御を受けていること、(2)同遺伝子座がコードするNB-LRRクラス抵抗性タンパク質の病原体認識にはLRRドメインが関わっていること、(3)同抵抗性タンパク質による認識される病原体分子(非病原力因子)の高次構造を明らかにした。これらの知見は抵抗性対立遺伝子座RCY1、HRT、RPP8による病害抵抗性の分子基盤の理解と、新規な病害防除方法への応用に寄与するものと考えられる。

Report

(6 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report

Research Products

(35 results)

All 2019 2018 2017 2016 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (9 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 9 results,  Open Access: 2 results,  Acknowledgement Compliant: 3 results) Presentation (22 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results) Book (2 results)

  • [Int'l Joint Research] ワーゲニンゲン大学(オランダ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] カフルエルシェイク大学(エジプト)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] First identification and characterization of cucumber mosaic virus from Corchorus olitorius in Japan2018

    • Author(s)
      Hanbal Sara E.、Miyashita Shuhei、Ando Sugihiro、Sidaros Samir A.、Takahashi Hideki
    • Journal Title

      Journal of Plant Pathology

      Volume: 100 Pages: 561-565

    • DOI

      10.1007/s42161-018-0099-6

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Survey of the response of 82 domestic landraces of Zea mays to cucumber mosaic virus reveals geographical region-related resistance to CMV in Japan2018

    • Author(s)
      Takahashi H.、Tian A.、Miyashita S.、Kanayama Y.、Ando S.、Kormelink R.
    • Journal Title

      Plant Pathology

      Volume: in press Pages: 1401-1415

    • DOI

      10.1111/ppa.12848

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Increased cytosine methylation at promoter of the NB-LRR class R gene RCY1 correlated with compromised resistance to cucumber mosaic virus in EMS-generated src mutants of Arabidopsis thaliana2017

    • Author(s)
      Sato Yukiyo、Miyashita Shuhei、Ando Sugihiro、Takahashi Hideki
    • Journal Title

      Physiological and Molecular Plant Pathology

      Volume: 100 Pages: 151-162

    • DOI

      10.1016/j.pmpp.2017.09.007

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] L-Histidine induces resistance in plants to the bacterial pathogen Ralstonia solanacearum partially through the activation of ethylene signaling.2016

    • Author(s)
      Seo, S., Nakaho, K., Hong, S-W., Takahashi, H., Shigemori, H. and Mitsuhara, I.
    • Journal Title

      Plant Cell Physiology

      Volume: 57 Pages: 1932-1942

    • DOI

      10.1093/pcp/pcw114

    • NAID

      120006771684

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of NtERF3 in the cell death signalling pathway mediated by SIPK/WIPK and WRKY1 in tobacco plants.2015

    • Author(s)
      Ogata, T., Okada, H., Kawaide, H., Takahashi, H., Seo, S., Mitsuhara, I. and Matsushita, Y.
    • Journal Title

      Plant Biology

      Volume: 17 Pages: 962-972

    • DOI

      10.1111/plb.12349

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cauliflower mosaic virus Tav protein induces leaf chlorosis in transgenic tobacco through a host response to its virulence function.2015

    • Author(s)
      Waliullah S, Kosaka N, Yaeno T, Ali ME, Sekine KT, Atsumi G, Yamaoka N, Nishiguchi M, Takahashi H and Kobayashi K
    • Journal Title

      Journal of General Plant Pathology

      Volume: - Pages: 261-270

    • DOI

      10.1007/s10327-015-0600-4

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of intron-mediated enhancement on accumulation of an Arabidopsis NB-LRR class R-protein that confers resistance to Cucumber mosaic virus.2014

    • Author(s)
      Sato, Y., Ando, S. and Takahashi, H.
    • Journal Title

      PLoS ONE

      Volume: 9

    • DOI

      10.1371/journal.pone.0107185

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Transcriptional profile of tomato roots exhibiting Bacillus thuringiensis- induced resistance to Ralstonia solanacearum.2014

    • Author(s)
      Takahashi, H., Nakaho, K., Ishihara, T., Ando, S., Wada, T., Kanayama, Y., Asano, S., Yoshida, S., Tsushima, S. and Hyakumachi, M.
    • Journal Title

      Plant Cell Reports

      Volume: 33 Pages: 99-110

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] WRKY70 interacting with RCY1 disease resistance protein is required for resistance to Cucumber mosaic virus in Arabidopsis thaliana.2014

    • Author(s)
      Ando, S., Obinata, A. and Takahashi, H.
    • Journal Title

      Physiological and Molecular Plant Pathology

      Volume: 85 Pages: 8-14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] エジプト産ササゲ品種EGのキュウリモザイクウイルス高度抵抗性を決定するウイルス因子の解析2019

    • Author(s)
      村山友理・井尾美由記・安藤杉尋・宮下脩平・Sara E. Hanbal・高橋英樹
    • Organizer
      平成31年度日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] キュウリモザイクウイルス(CMV)に対するエジプト産ササゲ品種EGの応答とCMVに対する高度抵抗性の性状解析2019

    • Author(s)
      井尾美由記・村山友理・安藤杉尋・宮下脩平・Sara E. Hanbal・高橋英樹
    • Organizer
      平成31年度日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Analysis of genomic region in cucumber mosaic virus RNA1 which determines the induction of the non-HR cell death in reassorted CMV-inoculated leaves of Arabidopsis thaliana2019

    • Author(s)
      Ainan Tian・Shuhei Miyashita・Sugihiro Ando・Hideki Takahashi
    • Organizer
      平成31年度日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] BTH処理によるRNAサイレンシング関連因子AGO2のプライミングを制御する転写因子の探索2019

    • Author(s)
      安藤杉尋・小関彩恵子・宮下脩平・高橋英樹
    • Organizer
      平成31年度日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] キュウリモザイクウイルスに不顕性感染したシロイヌナズナにおける2bタンパク質の役割2019

    • Author(s)
      高橋英樹・田原 緑・宮下脩平・安藤杉尋・福原敏行
    • Organizer
      平成31年度日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Latent plant-virus interactions and their impact on plant stress tolerance.2018

    • Author(s)
      Hideki Takahashi
    • Organizer
      WUR-TU Plant Science Workshop 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Comparative analysis of two-type of cell death developed in cucumber mosaic virus-inoculated leaves of Arabidopsis thaliana2018

    • Author(s)
      Ainan Tian, Shuhei Miyashita, Sugihiro Ando, Hieki Takahashi
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] R遺伝子による認識を免れる変異型CMV CPのアラニンスキャニングによる探索2018

    • Author(s)
      西條悠希, 安藤杉尋, 高橋英樹, 宮下脩平
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] シロイヌナズナへの無病徴感染に関わるキュウリモザイクウイルスのゲノムRNAと宿主因子の解析2018

    • Author(s)
      吉澤 峻, 宮下脩平, 安藤杉尋, 福原敏行, 高橋英樹
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] キュウリモザイクウイルスに無病徴感染したシロイヌナズナにおけるRNA-seq解析2018

    • Author(s)
      高橋英樹, 田原 緑, 平山裕也, 宮下脩平, 安藤杉尋, 川野修一, 福原敏行
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] EMS変異誘発シロイヌナズナ系統におけるNB-LRRクラス抵抗性遺伝子RCY1プロモーター領域シトシンメチル化とCMV抵抗性レベル低下の相関2017

    • Author(s)
      佐藤有希代・宮下脩平・安藤杉尋・高橋英樹
    • Organizer
      平成29年度日本植物病理学会大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヒストン脱メチル化酵素変異体ldlにおけるキュウリモザイクウイルス抵抗性の解析2017

    • Author(s)
      小関彩恵子・宮下脩平・高橋英樹・安藤杉尋
    • Organizer
      平成29年度日本植物病理学会大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] The Dawn of Agriculture immunology2016

    • Author(s)
      Takahashi, H
    • Organizer
      Lorentz Center Workshop
    • Place of Presentation
      Lorentz Center, Leiden, The Netherlands
    • Year and Date
      2016-09-19
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] R遺伝子による細胞死誘導の回避に寄与するキュウリモザイクウイルス外被タンパク質の1アミノ酸変異はウイルスの全身移行能を喪失させる2016

    • Author(s)
      安藤由起・宮下脩平・安藤杉尋・高橋英樹
    • Organizer
      平成28年度日本植物病理学会大会
    • Place of Presentation
      岡山県、岡山市
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] 次世代シークエンサーを用いたイネ有機栽培育苗培土の微生物多様性解析2015

    • Author(s)
      高橋英樹・松下裕子・伊藤豊彰・小林 隆・橋本知義・宮下脩平・守川俊幸・吉田重信・對馬誠也・安藤杉尋
    • Organizer
      平成27年度日本植物病理学会東北部会
    • Place of Presentation
      宮城県、仙台市
    • Year and Date
      2015-09-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 異なる有機栽培育苗土の微生物コミュニティーによるイネもみ枯細菌病抑制効果の比較2015

    • Author(s)
      安藤杉尋・鎗山純・小林隆・吉田重信・伊藤豊彰・宮下脩平・高橋英樹
    • Organizer
      平成27年度日本植物病理学会東北部会
    • Place of Presentation
      宮城県、仙台市
    • Year and Date
      2015-09-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] トマトモザイクウイルスのゲノム/サブゲノム合成量比を制御するcisエレメント2015

    • Author(s)
      宮下脩平・安藤杉尋・高橋英樹・石川雅之
    • Organizer
      平成27年度日本植物病理学会東北部会
    • Place of Presentation
      宮城県、仙台市
    • Year and Date
      2015-09-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] Molecular basis of RCY1/RPP8 locus conferred dual resistance to Cucumber mosaic virus and Hyaloperonospora arabidopsidis.2014

    • Author(s)
      Takahashi, H., Sato, Y., Ando, Y. and Ando, S.
    • Organizer
      3rd Korea-Japan Joint Symposium on Plant Pathology
    • Place of Presentation
      Busan, Korea
    • Year and Date
      2014-10-23 – 2014-10-24
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Analysis of pathogen-recognition domain in NB-LRR class RCY1/RPP8 locus conferring dual resistance to Cucumber mosaic virus and Hyaloperonospora arabidopsidis in Arabidopsis thaliana.2014

    • Author(s)
      Takahashi, H., Ando, Y. and Ando, S.
    • Organizer
      XVI International Congress on Molecular Plant-Microbe Interactions
    • Place of Presentation
      Rhodos, Greece
    • Year and Date
      2014-07-06 – 2014-07-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Role of intron-mediated enhancement on accumulation of an Arabidopsis NB-LRR class R-protein that confers resistance to Cucumber mosaic virus.2014

    • Author(s)
      Sato, Y., Ando, S. and Takahashi, H.
    • Organizer
      XVI International Congress on Molecular Plant-Microbe Interactions
    • Place of Presentation
      Rhodos, Greece
    • Year and Date
      2014-07-06 – 2014-07-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] キュウリモザイクウイルス・アブラナ科べと病菌複合抵抗性を支配するRCY1/RPP8遺伝子座の組換えと病原体応答2014

    • Author(s)
      高橋英樹・安藤杉尋
    • Organizer
      平成26年度日本植物病理学会大会
    • Place of Presentation
      札幌市
    • Year and Date
      2014-06-04 – 2014-06-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] キュウリモザイクウイルス抵抗性遺伝子RCY1に対するウイルス非病原性因子の機能ドメイン解析2014

    • Author(s)
      安藤由起・安藤杉尋・高橋英樹
    • Organizer
      平成26年度日本植物病理学会大会
    • Place of Presentation
      札幌市
    • Year and Date
      2014-06-04 – 2014-06-06
    • Related Report
      2014 Annual Research Report
  • [Book] Antiviral Resistance in Plants: Methods and Protocols, In Methods in Molecular Biology (Eds. Kappei Kobayashi and Masamichi Nishiguchi)2019

    • Author(s)
      Yukiyo Sato and Hideki Takahashi
    • Total Pages
      278
    • Publisher
      Springer
    • Related Report
      2018 Annual Research Report
  • [Book] Cucumber Mosaic Virus (Eds. Peter Palukaitis and Fernando Garcia-Arenal)2018

    • Author(s)
      Chikara Masuta and Hideki Takahashi
    • Total Pages
      380
    • Publisher
      APS Press
    • ISBN
      9780890546093
    • Related Report
      2017 Annual Research Report

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Published: 2014-04-04   Modified: 2020-03-30  

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