Study on the interaction between target cells and polyphenol conjugates underlying the molecular mechanism of their biological activity
Project/Area Number |
26292069
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Food science
|
Research Institution | The University of Tokushima (2015-2016) Nagoya University (2014) |
Principal Investigator |
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
|
Keywords | ポリフェノール / 抱合体 / マクロファージ / 標的細胞 / 標的分子 / 炎症 / フラボノイド / 標的タンパク質 / 代謝 |
Outline of Final Research Achievements |
We first examined the anti-inflammatory effects of a quercetin glucuronide (Q3GA) and a sulfate (Q3'S) in macrophages. Next, to identify the target molecules of quercetin, a major polyphenol in human diet, and of its glucuronide metabolite (Q3GA), the derivatives of quercetin and Q3GA were chemically synthesized. We also developed a reaction condition for the formation of cross-link between proteins and these quercetin derivatives. On the other hand, we developed an analytical condition of LC-MS/MS for detection of quercetin metabolites in human plasma. High-sensitive LC-MS/MS analysis enables us to investigate the effects of different methods for quercetin intake on the metabolic profiles of quercetin in humans.
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Report
(4 results)
Research Products
(11 results)